FIGURE 1.

Growth and phenotypic comparisons of wild-type (4A+), 2pac, two complemented lines (gRBD1–1 and gRBD1–2) and a strain lacking the psbA gene encoding the D1 protein (Fud7). A, growth (upper panels) and variable fluorescence (F_v/F_m, lower panels) of strains grown on plates with (TAP) and without (HS) acetate at 30 μmol photons m⁻² s⁻¹. B, immunoblot analysis of steady-state levels of PSII (D1, D2, and CP43), PSI (PsaA and PsaD), cytochrome b₆f (Cyt f), and ATP synthase (AtpB) subunits, as well as of RBD1. C, model of the RBD1 protein showing predicted N-terminal chloroplast transit peptide (green), rubredoxin domain (red, with locations of conserved iron-coordinating cysteines denoted by yellow triangles) and C-terminal transmembrane helix (blue). D, phase a component of A_{520 nm}, a measurement of the electrochemical gradient formed after illumination, before (black) and after (white) treatment with the PSII-specific inhibitors DCMU and HA. E, P700 oxidation and reduction kinetics of wild type (WT) and 2pac, measured by absorbance at 705 nm after treatment with DCMU and HA.