FIGURE 4.
Characterization of the ΔrubA mutant of the cyanobacterium Synechocystis sp. PCC 6803. A, genotyping of the mutant strains was performed via PCR amplification of both the flanking region of rubA (using primers 1 and 2), the rubA coding sequence (CDS, using primers 3 and 4) and neutral site used for RubA overexpression (slr0168, using primers 5 and 6) from the wild type (WT), ΔrubA mutant and RubA OE strain. B, growth (upper panels) and variable fluorescence (Fv/Fm, lower panels) of wild type (WT), ΔrubA and a complemented line (RubA OE) grown on plates with and without glucose. C, immunoblot analysis of WT, ΔrubA, and RubA OE. D, P700 oxidation and reduction kinetics measured on equal numbers of cells of WT and ΔrubA mutant.