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. 2013 Aug 5;288(37):26775–26784. doi: 10.1074/jbc.M113.483743

FIGURE 2.

FIGURE 2.

PP2Ac does not induce Th17-associated transcription factors. A, naïve CD4 T cells from WT or PP2Ac transgenic mice were stimulated for the indicated time periods. Il17a mRNA was normalized to Actb levels (mean ± S.E.). *, p ≤ 0.01. B, microarray expression values of the indicated genes are shown. C, naïve CD4 T cells were stimulated overnight. Expression of the indicated genes was normalized to Actb. Shown is the fold expression relative to WT cells (mean ± S.E.). D, naïve CD4 T cells were stimulated during 48 h, expanded with IL-2 during 5 days, rested 48 h, washed and incubated with IL-6 for 3 h. Cells were lysed in radioimmune precipitation assay buffer in the presence of protease and phosphatase inhibitors. E, naïve CD4 T cells were stimulated with anti-CD3 and anti-CD28 and lysed at the indicated time points. Data are representative of at least four experiments, each with ≥ 3 mice/group (A and C), or ≥ 2 experiments, each performed with cells pooled from ≥ 3 mice (D and E).