FIGURE 3.

The PP2Ac effect is independent of T cell effector differentiation. A, naïve CD4 T cells were stimulated in neutral (Th0) or Th17-inducing conditions (TGF-β1 and IL-6). At day 5, expression of the indicated genes was analyzed by real-time PCR. Results were normalized to Actb. Shown is the fold expression relative to WT Th0 cells (mean ± S.E.). B, naïve CD4 T cells were stimulated in Th1- (IL-12) or Th17-polarizing conditions. After 5 days, expression of Ifng and Il17a was analyzed by real-time PCR. Results are expressed as fold expression relative to Actb. Data are representative of four (A) or three (B) experiments, each with three mice per group.