FIGURE 4.

PP2Ac increases chromatin accessibility at the Il17 locus. A, the mouse Il17 locus is depicted. The conserved non-coding sequences as well as the sites within the Il17a and Il17f genes that were amplified during the ChIP experiments are indicated. B, naïve CD4 T cells were stimulated and fixed, and ChIP was performed for acetylated H3, H3K9me3, H3K4me3, and H3K27me3. Shown is relative binding (PP2Ac:WT ratio) in resting (blue) or stimulated (red) cells. Data were normalized to the corresponding input DNA and are representative of three or four independent experiments, each with ≥ 3 mice/group. PrPr, proximal promoter.