FIGURE 5.

Protein synthesis is not required for sFlt-1 transcription, but NF-κB is necessary for translation. A, PBMs were pretreated with cycloheximide, activated as above, then transcripts of sFlt-1 were measured using real-time RT-PCR (representative donor; n ≥ 3). B, PBMs were pretreated with cycloheximide and activated as above, then ELISAs were performed to measure sFlt-1 in the supernatants (representative donor; n ≥ 3). C, PBMs were pretreated with or without BAY 11-7085, then FcγR clustered as described above. ELISAs were done to measure sFlt-1 in supernatants (representative donor; n ≥ 3). D, PBMs were pretreated and activated as in A, then the transcript of sFlt-1 was measured using real-time RT-PCR. (representative donor; n ≥ 3).