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. 2013 Aug 2;288(37):26810–26819. doi: 10.1074/jbc.M113.497032

FIGURE 3.

FIGURE 3.

In vitro analysis of linker-deleted Vps4 proteins. A, SDS-PAGE analysis of the recombinant Vps4 proteins used in the in vitro assays. Proteins are visualized by Coomassie Blue staining. B–D, Vps4 ATPase activity in the absence (B) or presence of the substrate Did2 (C) or the co-factor Vta1 (D). E, in vitro ESCRT-III disassembly by wild-type and mutant Vps4. Error bars in panels B–E indicate S.D.