FIGURE 5.
Constitutive activation of SOCE leads to accumulation of APP β-CTFs. A, Western blot analysis of full-length APP and APP CTFs in HEK-APP vector and HEK-APP-Orai1 cells transfected with control, YFP-STIM1, or YFP-STIM1D76A plasmids is shown. B, quantification of α- and β-CTF levels, normalized to loading control (Flot-2) HEK-APP-vector cells transfected with control plasmid is shown. C, Western blot analysis of transfected HEK-APPswe and HEK-APPswe-Orai1 cells is as described in A. D, quantification of α- and β-CTF levels is as described in B. Data represent three experiments performed in duplicate; one-way analysis of variance; *, p < 0.05; **, p < 0.01; ***, p < 0.001.