FIGURE 7.
Modulation of SOCE alters levels of secreted Aβ in HEK-APP and HEK-APPswe cells. A and B, HEK-APP-vector and HEK-APP-Orai cells were transiently transfected with control plasmid, YFP-STIM1, or YFP-STIM1D76A. The levels of Aβ40, Aβ42 (A), and sAPPα (B) in conditioned media were quantified by ELISA. C and D, HEK-APPswe-vector and HEK-APPswe-Orai1 cells were transfected as above, and the levels of secreted Aβ40, Aβ42, and sAPPα were analyzed by ELISA. Data represent three experiments performed in duplicate; one-way analysis of variance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. E, HEK cells stably expressing Orai1myc were co-transfected with C99–6myc and either empty vector or YFP-STIM1D76A. Western blot analysis of C99 and AICD was then performed using an anti-myc antibody (9E10), and the ratio of AICD:C99 was quantified; n = 8 transfections; Student's t test; **, p < 0.01.