Table 4.
Expression of members of the vacuolar iron transporter (VIT) family encoded in the soybean genome.
| Transcriptome | TMD | Homology to AtVit1 (% similarity) | Homology to LjSEN1 (% similarity) | ||||
|---|---|---|---|---|---|---|---|
| Severin et al. (2010) | Libault et al. (2010) | ||||||
| Root | Nodule | Root | Nodule | ||||
| Glyma01g36530 | 0 | 0 | 0 | 0 | 4 | 21 | 52 |
| Glyma02g09110 | 18 | 1 | 2 | 19 | 4 | 22 | 51 |
| Glyma05g24980 | 0 | 0 | 0 | 2 | 5 | 19 | 52 |
| Glyma05g24990 | 0 | 0 | 0 | 1 | 5 | 20 | 54 |
| Glyma05g25000 | 0 | 1 | 8 | 0 | 4 | 20 | 65 |
| Glyma05g25010 | 0 | 761 | 16 | 2134 | 5 | 19 | 65 |
| Glyma05g34430 | 5 | 8 | 3 | 6 | 5 | 81 | 22 |
| Glyma08g05230 | 5 | 3 | 10 | 2 | 5 | 82 | 22 |
| Glyma08g08070 | 10 | 0 | 0 | 8 | 4 | 12 | 36 |
| Glyma08g08090 | 6 | 1 | 0 | 17 | 4 | 19 | 54 |
| Glyma08g08100 | 3 | 0 | 0 | 8 | 5 | 19 | 54 |
| Glyma08g08110 | 0 | 0 | 3 | 0 | 4 | 21 | 63 |
| Glyma08g08120 | 0 | 192 | 3 | 801 | 4 | 21 | 62 |
| Glyma08g19390 | 3 | 0 | 1 | 7 | 4 | 19 | 52 |
| Glyma10g37030 | 1 | 0 | 0 | 1 | 4 | 24 | 57 |
| Glyma11g08830 | 0 | 0 | 0 | 1 | 5 | 20 | 54 |
| Glyma15g05610 | 0 | 0 | 0 | 1 | 3 | 19 | 50 |
| Glyma16g28340 | 21 | 4 | 9 | 61 | 4 | 22 | 52 |
| Glyma18g46245 | 0 | 0 | – | – | 5 | 22 | 17 |
| Glyma20g30580 | 0 | 3 | – | – | 4 | 21 | 55 |
A comparison of each transporter's expression in root and nodule tissue from two soybean transcriptome studies is presented (Libault etal., 2010; Severin etal., 2010). A prediction of the number of transmembrane domains (TMD) each protein has was analyzed by SOSUI (http://bp.nuap.nagoya-u.ac.jp/sosui/sosui_submit.html). Homology to two VIT family members, AtVIT1, a characterized ferrous iron transporter and LjSEN1, which is essential for nitrogen fixation in L. japonicus, were calculated by a clustalW alignment.