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. 2013 Sep 13;7:151. doi: 10.3389/fncel.2013.00151

FIGURE 1.

FIGURE 1

Conditional ablation of Dicer in postmitotic neurons of the cerebral cortex results in a smaller cortex and reduced thickness of the cortical wall. (A) Dicer targeting construct. The N-terminal RNA helicase domain, piwi argonaute and zwille (PAZ) domain, two ribonuclease III domains, and a double-stranded RNA- binding domain (RBD) are labeled. The exon 22 and exon 23 of Dicer are conditionally excised after Nex-Cre recombination. (B) X-gal staining at the level of the cortex in a Nex-Cre and Rosa26-LacZ- expressing mouse at P1 illustrating cortical specificity of the Nex-Cre line. The red box indicates the region shown at higher magnification. The cortex (Cx) and hippocampus (Hp) are labeled. (C) Appearance of representative brains from P10 control and Nex-Cre-Dicer Ko mice (litter mates). (D) Body and brain weights of control (Ctrl) and Nex-Cre-Dicer Ko (Ko) mice at P10. (E) Coronal sections of P10 brains with Nissl staining of control and Nex-Cre-Dicer Ko mice. The black boxes indicate the region shown at higher magnification. The subplate (SP), cortical plate (CP), and marginal zone (MZ) are labeled. (F) In situ hybridization of miR-9, Let-7, and miR-128 in control and Dicer Ko cortices at P10. Data are presented as mean ± SEM; n ≥ 3 in all genotypes; p values in relation to control (*p < 0.05, ***p < 0.00002).