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. 2013 Sep 13;7:151. doi: 10.3389/fncel.2013.00151

FIGURE 2.

FIGURE 2

Conditional Dicer loss in mature neurons does not significantly alter cortical lamination and neuronal production, and exhibits a transitory increase in cell death at P5. (A,B) The numbers of early-born neurons labeled with Tbr1 and Ctip2 were unaffected in P5 Nex-Cre-Dicer Ko (Ko) cortices, compared to controls (Ctrl). The numbers of late-born neurons labeled with Satb2 were unaffected and Cux1 were decreased in P5 Dicer Ko cortices, compared to controls. (C,D) Numbers of NeuN+ and DAPI+ cells were unaffected in the cortical wall of P5 Dicer Ko cortices compared to controls. The dashed box indicates the area of quantification. (E) TUNEL assay of coronal cryosections of P5 control and Dicer Ko cortices. The dashed box indicates the region shown at higher magnification in panel a′, b′. (F) Normalized quantification of TUNEL+ cells per area in the cortical wall of P5 control and Dicer Ko brains. Cortical layers (VI), (V), and (II/III) are labeled. Scale bar: 100 μm. Data are presented as mean ± SEM; n ≥ 3 in all genotypes; p values in relation to control (*p < 0.04, ***p < 0.0002). n.s., not significant.