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. 2013 Sep 13;7:151. doi: 10.3389/fncel.2013.00151

FIGURE 3.

FIGURE 3

Depletion of miRNA function in postmitotic neurons slightly alters early-born neurons and has no measured effect on neuronal production and cell death at P10. (A,B) The numbers of early-born neurons labeled with Tbr1 were increased and Ctip2 were decreased in P10 Nex-Cre-Dicer Ko (Ko) cortices, compared to controls (Ctrl). The numbers of late-born neurons labeled with Satb2 and Cux1 were unaffected in P10 Dicer Ko cortices, compared to controls. (C,D) Numbers of NeuN+ and DAPI+ cells were unaffected in the cortical wall of P10 Dicer Ko cortices compared to controls. The dashed box indicates the area of quantification. (E) TUNEL assay of coronal cryosections of P10 control and Dicer Ko cortices. Arrows indicate TUNEL+ cells. (F) Normalized quantification of TUNEL+ cells per area in the cortical wall of P10 control and Dicer Ko brains. Cortical layers (VI), (V), and (II/III) are labeled. Scale bar: 100 μm. Data are presented as mean ± SEM; n ≥ 3 in all genotypes; p values in relation to control (*p < 0.04). n.s., not significant.