FIGURE 4.

Conditional Dicer ablation increases neuron and cell packing density within the cortex at P10. (A,B) Numbers of NeuN⁺ and DAPI⁺ cells were unaffected in the upper and lower regions of the cortical wall of P5 Nex-Cre-Dicer Ko (Ko) cortices compared to controls (Ctrl). (C,D) Numbers of NeuN⁺ and DAPI⁺ cells in the upper and lower regions of the cortical wall were significantly increased in P10 Dicer Ko cortices compared to controls. The dashed boxes indicate the region shown at higher magnification in panel i′, i^′′, ii′, ii^′′; the boxed area in this region was chosen for subsequent analysis. The ventricular surface (vs) and pial surface (ps) are labeled. Scale bar: 100 μm. Data are presented as mean ± SEM; n ≥ 3 in all genotypes; p values in relation to control (*p < 0.02, **p < 0.004, ***p < 0.0008). n.s., not significant.