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. 2013 Sep 13;7:151. doi: 10.3389/fncel.2013.00151

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Copyright © Hong, Zhang, Kawase-Koga and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Loss of Dicer in mature neurons delays neurite outgrowth in vitro. (A) An illustrative summary of primary neuronal culture derived from control (Ctrl) and Nex-Cre-Dicer Ko (Ko) P0 dorsal cortex. (B) Measurements of Map2⁺ processes revealed shorter neurite outgrowth 10 days in vitro (DIV 10) in Dicer Ko (N = 113) neural cultures compared to controls (N = 158). (C) Measurements of Tuj1⁺ processes displayed shorter neurite outgrowth in DIV 10 Dicer Ko (N = 411) neural cultures compared to controls (N = 423). Scale bar: 50 μm. Data are presented as mean ± SEM; n ≥ 3 in all genotypes; p values in relation to control (***p < 0.0008).