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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Methods Mol Biol. 2009;520:39–58. doi: 10.1007/978-1-60327-811-9_4

Figure 1.

Figure 1

The detection of glycans on proteins captured by antibody arrays. The drawing depicts antibodies immobilized on a planar surface. The glycans on the antibodies are derivatized to prevent lectin binding; a sample is incubated on the antibody array; proteins are captured by the antibodies; biotinylated lectins bind to the glycans on the captured proteins; and the level of bound lectin is determined by scanning for fluorescence from streptavidin-B-phycoerythrin.