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. 2013 Sep 15;126(18):4239–4252. doi: 10.1242/jcs.130195

Fig. 1.

Fig. 1.

The subunits of the SMC5/6 complex are expressed in the first wave of spermatogenesis, beginning when zygotene spermatocytes appear. (A) PCR analysis of mRNA expression of SMC5/6 complex subunits Smc5, Smc6, Nsmce1-4b during the first wave of spermatogenesis, from mice aged 4, 7, 10, 13, 16, 19, 22 and 56 (adult) d.p.p. Sycp1 was used as a control for progression of the first wave of spermatogenesis and Actb was used as an mRNA loading control. (B) Western blot analysis of protein expression of SMC5/6 complex subunits SMC5 (GeneTex), SMC6 (Abcam), SMC6 (Lehmann), NSMCE1, NSMCE4A and NSMCE4B during the first wave of spermatogenesis, from mice aged 7, 10, 13, 16, 19, 22 and 56 (adult) d.p.p. SYCP1 was used as a control for progression of the first wave of spermatogenesis and α-tubulin (TUBA) was used as a protein loading control.