Fig. 9.
The distribution of SMC5 at the synaptonemal complex central region and SMC6 Ab at chromocenters is independent of SMC1β-containing cohesin axes. Triple immunolabeling of SMC5 Ge and SMC6 Ab (green), SYCP3 (red) and TEX12 (light blue) or γH2AX (light blue) on spread Smc1β+/+ wild-type pachytene (A,C,E,G) and Smc1βrepro11 pachytene-like (B,D,F,H) mutant spermatocytes. The sex body (XY) and sex univalents (X,Y) are indicated. Boxed areas in the merged images are enlarged in the insets at the right column. Arrowheads in B,D indicate asynapsed AEs. Scale bar: 10 µm.