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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Environ Microbiol. 2012 Sep 9;15(3):895–906. doi: 10.1111/j.1462-2920.2012.02867.x

Fig. 4.

Fig. 4

Heterologous expression of olsC from Rhizobium tropici in Agrobacterium tumefaciens A208. Lipids of the wild-type A. tumefaciens A208 harbouring the empty plasmid pERMAV06 (A) or the olsC-containing plasmid pERMAV15 (B) were labelled with [14C]acetate for 48 h during growth in LB medium at 30°C and separated using two-dimensional TLC. The phospholipids phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), cardiolipin (CL) and the OLs S1, and S2 are indicated. OLs P1 and P2 derived from OlsC-dependent hydroxylation of S1 and S2 are also indicated.