Table 2.
Membrane lipid composition of A. tumefaciens wild-type, olsE-deficient mutant MAV07 and olsB-deficient mutant MAV08 after labelling for 48 h with [14C]acetate in Sherwood minimal medium at 30°C or 15°C.
| Composition (% of total 14C) | ||||||
|---|---|---|---|---|---|---|
|
| ||||||
| 30°C | 15°C | |||||
|
|
|
|||||
| Lipid | A208 | MAV07 | MAV08 | A208 | MAV07 | MAV08 |
| PC | 13.6 | 14.7 | 19.3 | 28.4 | 34.1 | 33.3 |
| S1 | 6.8 | 20.2 | n.d. | n.d. | 16.0 | n.d. |
| S2 | 14.3 | n.d. | n.d. | 16.8 | n.d. | n.d. |
| PE | 29.7 | 30.8 | 43.0 | 39.0 | 31.3 | 52.6 |
| PG | 12.2 | 11.5 | 18.9 | 11.6 | 15.0 | 11.7 |
| CL | 23.4 | 22.8 | 18.8 | 4.2 | 3.6 | 2.4 |
The values shown are mean values derived from at least two independent experiments.
S1: unmodified ornithine lipid; S2: hydroxylated ornithine lipid; n.d.: not detected.