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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Dev Biol. 2013 Jun 21;381(2):401–410. doi: 10.1016/j.ydbio.2013.06.022

Fig. 1.

Fig. 1

Tamoxifen administration activates Atoh1CreERT2 in nearly all cochlear hair cells and efficiently deletes an Atoh1flox allele. (A) Cochlea from E18.5 Atoh1CreERT2/+; ROSALacZ embryo treated with tamoxifen at E15.5 and immunostained for β-galactosidase. Box shows location of inset. Nearly every hair cell is labeled. (B) RT-PCR for Atoh1 on organ of Corti total RNA isolated from Atoh1CreERT2/flox verifies Atoh1 deletion. (C–F) In situ hybridization for Atoh1 on cochleae (C, D) and vestibular organs (E, F) from E18.5 control (C, C′, and E) and Atoh1CreERT2/flox (D, D′, and F) embryos following tamoxifen administration at E16.5. No labeling is present in the Atoh1CreERT2/flox embryonic cochlea or vestibular system. Boxes show locations of C′ and D′; brackets identify organ of Corti. AC – anterior crista, HC – horizontal crista. Scale bar: 500 μm (A); 100 μm (C–F); 25 μm (C′, D′).