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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Dev Biol. 2013 Jun 21;381(2):401–410. doi: 10.1016/j.ydbio.2013.06.022

Fig. 6.

Fig. 6

Atoh1 deletion at E16.5 abolishes cochlear expression of Barhl1 and Gfi1 but not several other genes. Pregnant dams received tamoxifen at E16.5 and embryos were harvested at E18.5. (A) Qualitative RT-PCR of organ of Corti mRNA from control and Atoh1CreERT2/flox embryos demonstrates that expression of Barhl1 and Gfi1 is lost in Atoh1CreERT2/flox mice. Upstream specification factors, hair cell markers, supporting cell markers and Pou4f3 continue to be expressed. (B, C) In situ hybridization for Barhl1 confirms absence of expression in Atoh1CreERT2/flox embryonic cochleae. (D–E′″) Pou4f3 continues to be expressed in all regions of Atoh1CreERT2/flox embryonic cochleae where hair cells are present. Pou4f3 expression is also maintained in the vestibular system (brackets). Scale bar: 100 μm.