Table 2.
Utricular hair cells require Atoh1 for survival and stereocilia formation. Total numbers of immunostained cells were counted in the indicated number of utricles harvested from E18.5 embryos and reported as average number ± SEM. Reported decreases are relative to littermate controls. Values for E14.5 and E16.5 tamoxifen administration are compared within genotype at the bottom of the Table.
| Control littermate | Atoh1CreERT2/flox | Decrease | p-value (t-test) | |
|---|---|---|---|---|
| Tamoxifen at E14.5 | n=4 utricles | n=4 utricles | ||
| Myosin VIIa+ | 1559 ± 17 | 881 ± 56 | 43% | 2.5 × 10−5 |
| Phalloidin+hair bundle | 965 ± 25 | 394 ± 17 | 59% | 1.4 × 10−6 |
| % Phalloidin+/Myosin VIIa+ | 62 ± 1% | 45 ± 1% | 17% | 8.4 × 10−5 |
| Tamoxifen at E16.5 | n=4 utricles | n=4 utricles | ||
| Myosin VIIa+ | 1525 ± 20 | 1076 ± 36 | 29% | 3.6 × 10−5 |
| Phalloidin+hair bundle | 946 ± 24 | 544 ± 23 | 42% | 2.1 × 10−5 |
| % Phalloidin+/Myosin VIIa+ | 62 ± 1% | 51 ± 3% | 11% | 0.014 |
| Tamoxifen E14.5 vs. E16.5 | p-value (t-test) | p-value (t-test) | ||
| Myosin VIIa+ | 0.25 | 0.03 | ||
| Phalloidin+hair bundle | 0.60 | 0.0020 | ||
| % Phalloidin+/Myosin VIIa+ | 0.97 | 0.13 |