Table 3.
Atoh1CreERT2/fox embryonic utricles have regionally-variable differentiation defects but no change in cell density. Pregnant dams received tamoxifen at E14.5 and embryos were harvested at E18.5. Total numbers of DAPI+ nuclei in the regions of interest (450−500 cells; see Methods) were counted for cell density calculations (DAPI+nuclei/100 μm2). Hair bundles were qualitatively scored as long, short or absent. All numbers are reported as average ± SEM; percent change is relative to littermate controls.
| Control littermate | Atoh1CreERT2/flox | % Change | p-value (t-test) | |
|---|---|---|---|---|
| DAPI+ nuclei/100 μm2 | n=2 utricles | n=2 utricles | ||
| Central | 7.1 ± 0.2 | 7.1 ± 0.3 | 0% | 0.97 |
| Peripheral | 7.1 ± 0.3 | 6.8 ± 0.2 | −4% | 0.43 |
| Myosin VIIa+/DAPI+ | n=4 utricles | n=4 utricles | ||
| Central | 74 ± 4% | 61 ± 3.8% | −13% | 0.04 |
| Peripheral | 70 ± 3.8% | 43 ± 3.2% | −27% | 3.4 × 10−4 |
| Hair bundles (phalloidin+/myosin VIIa+) | n=4 utricles | n=4 utricles | ||
| Long | ||||
| Central | 29 ± 1.6% | 27 ± 5.9% | −2% | 0.70 |
| Peripheral | 4.9 ± 2.1% | 8.2 ± 2.1% | +3.3% | 0.27 |
| Short | ||||
| Central | 36 ± 1.6% | 23 ± 3.3% | −13% | 0.003 |
| Peripheral | 50 ± 3.9% | 22 ± 2.5% | −28% | 6.6 ×10−6 |
| Absent | ||||
| Central | 35 ± 1.6% | 50 ± 3.4% | +15% | 6.7 × 10−4 |
| Peripheral | 45 ± 4.0% | 69 ± 2.6% | +24% | 4.5 × 10−5 |