Atoh1: 94 °C (30 s), 60 °C (30 s), 72 °C (60 s) for 10 cycles

94 °C (30 s), 50 °C (30 s), 72 °C (60 s) for 30 cycles

All other genes: 95 °C (45 s), 58 °C (45 s), 72 °C (90 s) for 40 cycles.