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Potential epigenetic pathways in ants. (A) Ratio of 5-methyl-cytosine/total DNA in C. floridanus and H. saltator, as determined by dot blot densitometry. Bars show mean + SEM; p value is from repeated measurement ANOVA (Wilks’ lambda = 0.52), n=10. (B–C) Quantification of Hsal_14941 (B) and Hsal_08142 (C) expression in different developmental stages and castes. White bars, qRT-PCR mean + SEM, n=7. Black bars, RNA-seq.
