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Intracellular ADA activity decreases with culture age. Long-term T cultures were established by activation with Ab-coated microbeads. CD8+ T lymphocytes were purified 4 d postsimulation, and cell lysates were assessed for ADA activity using the colorimetric assay described by Vielh and Castellazzi (30). Intracellular ADA activity for five individual cultures, all at equivalent PDs, is presented. **p , 0.01.
