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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: J Immunol. 2010 Feb 10;184(6):2847–2854. doi: 10.4049/jimmunol.0903647

FIGURE 3.

FIGURE 3

Surface ADA expression is reduced as human CD8 T cells approach replicative senescence. Cultures were established by activation with Ab-coated microbeads, and ADA expression was determined by flow cytometry 4 d after each stimulation (i.e., every ∼14 d). A, Histogram showing expression of ecto-ADA (determined by flow cytometry) on CD3+CD8+ cells from a representative culture at 8 PDs and 25 PDs. B, Mean (±SD) percentage of the CD8+ T cells that express ecto-ADA at early (8 PDs) and late (25 PDs) time points; the lines connect data from four individual cultures. *p < 0.05. C, ADA tran-scriptional expression (n = 4) normalized against GAPDH. **p < 0.01.