Table I.
CD8+CD28+ADA+/CD8+CD28+ADA- | |
---|---|
Donor | |
1 | 1.81 |
2 | 1.88 |
3 | 1.77 |
4 | 2.18 |
5 | 1.51 |
Mean ± SD | 1.83 ± 0.241 |
p value | 0.000057 |
PBMCs were stimulated with an irradiated allogeneic EBV-transformed B cell line as described in Materials and Methods. On day 4, purified CD8+ T cells, which were 97% (± 2%) CD28+, were separated into ADA+ and ADA− and purity verified by flow cytometry. The TRAP assay was performed on lysates from the equivalent of 2500 cells per lane. Data are expressed as the fold increase in telomerase activity of CD8+CD28+ADA+ over CD8+CD28+ADA− for each donor.