Figure 1. ADAP-deficient T cells show impaired antigen-dependent T cell activation in vivo.
CTV-labeled wild-type and ADAP−/− DO11.10 T cells (200,000 T cells each) were co-transferred into IFA/OVA primed hosts. (A) Proliferation in the draining LNs was assessed at different times. Proliferation (B) and PD-1 expression (D) was assessed at 48 hours post-transfer at different antigen concentrations. (C) CTV-labeled wild-type and ADAP−/− DO11.10 T cells (10,000 cells each) were co-transferred into IFA/OVA ear primed hosts. Clonal expansion in the draining LNs was assessed at the indicated times. All plots are each representative of at least 3 independent experiments. *, P<0.05 unpaired t test.
