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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Aug 5;191(5):2372–2383. doi: 10.4049/jimmunol.1300107

Figure 3. ADAP-deficient T cells do not form early stable contacts with cognate DCs in vivo.

Figure 3

CTV-labeled wild-type (blue) and CTO-labeled ADAP−/− (red) DO11.10 T cells (3 × 106 cells each) were co-transferred into CFSE/IFA ear-primed recipients, with or without 1 µg OVA antigen (Ag), and draining LNs were harvested four hours after transfer and imaged by TPLSM. Average track velocity (A) and cell displacement (B) of individual movies representative of at least 5 independent experiments. (C) Three-dimensional images and corresponding kymograph of a single CFSE-labeled DC (green) interacting with 2 wild-type (blue) and 2 ADAP −/− (red) DO11 T cells. (D) Kymograph of T-DC interactions with multiple DCs from a single acquired movie. (E) Pooled cumulative T-DC contact time at 4 hours after antigen challenge from three separate movies. (F) Pooled cumulative T-DC contact time at 4 hours after challenge with a high dose of antigen (5 µg) from three separate movies. (G) Pooled cumulative T-DC contact time at 8 hours after challenge with 1 µg OVA from three separate movies. *, P<0.05 unpaired t test.