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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Aug 5;191(5):2372–2383. doi: 10.4049/jimmunol.1300107

Figure 4. ADAP-deficient T cells are not confined near DC contact sites at the late swarming contact phase.

Figure 4

CTV-labeled wild-type (blue) and CTO-labeled ADAP−/− (red) DO11.10 T cells (1 × 106 cells each) were co-transferred into CFSE/IFA ear-primed recipients, with or without OVA antigen (Ag), and draining LNs were harvested 24 hours after transfer and imaged by TPLSM. (A) Average track velocity of individual movies from 3 independent experiments. (B) Pooled cumulative T cell-DC contact time from three independent experiments. (C) Three-dimensional images of CFSE-labeled DCs (green) interacting with wild-type (blue) and ADAP−/− (red) DO11.10 T cells. The images show the initial T cell-DC contact, the contact termination or post T cell-DC contact, and the endpoint displacement of the cell after 5 min or 20 min. (D) The pooled confinement ratio of the tracks described in (C) from 3 independent experiments. *, P<0.05; **, P=0.001 unpaired t test.