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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Aug 5;191(5):2372–2383. doi: 10.4049/jimmunol.1300107

Figure 5. The SKAP55 binding region in ADAP regulates early T cell activation in vivo.

Figure 5

Primary ADAP−/− DO11.10 hCAR T cells were transduced with control adenovirus expressing Thy1.1 or adenovirus expressing Thy1.1 and either wild-type ADAP or the ADAPΔ338 mutant as described in Methods. ADAP−/− reconstituted cells were co-transferred with wild-type DO11.10 hCAR T cells transduced with control adenovirus expressing Thy1.1 into OVA/IFA primed mice. (A) Proliferation in draining LNs was determined by CFSE (Ctrl + Thy; wild-type DO11.10 T cells transduced with control Thy1.1 adenovirus) and CTV (Experimental; ADAP−/− DO11.10 T cells transduced with the indicated adenovirus) dilution 48 hours after transfer. Cell samples were also stained for PD-1 expression. (B) Draining LNs were harvested at 4 hours after transfer, fixed and stained for phosphorylated c-Jun and S6. (C) Intracellular staining for phosphorylated c-Jun and S6 from 3 independent experiments as in (B) normalized to the maximum response in each mouse (response of wild-type DO11.10 T cells transduced with control adenovirus). *, P<0.05 unpaired t test.