Fig. 4. Internal standard calibration curves for the un-oxidized fatty acids and oxidized fatty acids analyzed using LC-MS/MS.
15(s)-HETE-d8 (12 fmol on column) was used as internal standard for preparing these standard calibration curves. Calibration range: 0 – 4 mol/mol, Panel A) 15(S)-HETE-d8 for HETEs, Panel B) EETs, oxoEETs, Panel C) HODEs, and oxoODEs, Panel D) 18 – 150 mol/mol 15(S)-HETE-d8 for linoleic acid (LA) and arachidonic acid (AA).
