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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2013 Mar 14;59:2–13. doi: 10.1016/j.freeradbiomed.2013.03.001

Fig. 6. Representative LC-MS/MS chromatogram of un-oxidized fatty acids and oxidized fatty acids extracted from human plasma.

Fig. 6

Extraction as described in “Materials and Methods”; separation was performed on a reverse phase C18 column (2.1 × 250mm, 5μm) using acidified methanol/water as mobile phase at flow rate of 0.2 ml/min using a gradient as described in “Materials and Methods”. Note that some of the oxidized fatty acid species have common MRM pairs and unique retention times;8,9-EET exhibits the same MRM transitions as both 8-HETE and 9-HETE, and 11,12-EET shares the same MRM transitions with both 11-HETE and 12-HETE. The unique retention times of the 8,9-EET and 11,12-EET were established by injection of authentic standards (data not shown).