Fig. 7. A representative LC-MS/MS chromatogram of PGF2α's extracted from human urine.

Top panel shows heavy isotope labeled internal standard PGF2α-d4 from urine second panel shows the corresponding transition for PGF2α's which are chromatographically resolved from their free radical derived isomers, the 8-Iso-PGF2α's. Bottom two panels show other free radical derived PGF2α isomers with different MRM transitions.