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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2013 Mar 14;59:2–13. doi: 10.1016/j.freeradbiomed.2013.03.001

Fig. 8. Dual High Pressure Mixed Binary Gradient Elution HPLC System.

Fig. 8

The plumbing schematic for the HPLC system used for high-throughput chromatography is shown. The valves are configured so that column 2 is in-line with the mass spectrometer while valve 3 serves as a bypass valve to waste, preventing effluent from reaching the mass spectrometer during any period of non-data collection.