Table 1.

Limit of Detection (LOD) and Limit of Quantitation (LOQ)

Analyte	Transition (m/z)	aLOD (pg)	bLOQ (ng/ml)
Arachidonic acid	303 → 259	91	3.1
5-HETE	319 → 115	4.8	0.16
8-HETE	319 → 155	4.8	0.1
9-HETE	319 → 151	9.6	0.30
11-HETE	319 → 167	2.6	0.09
12-HETE	319 → 179	3.2	0.11
15-HETE	319 → 175	13	0.45
5,6-EET	319 → 191	16	0.54
8,9-EET	319 → 151	19	0.64
11,12-EET	319 → 167	16	0.54
14,15-EET	319 → 175	26	0.90
5-oxoETE	317 → 203	32	1.1
12-oxoETE	317 → 153	32	1.1
15-oxoETE	317 → 113	64	2.2
PGF2α	353 → 193	7.1	0.24
Linoleic acid	279 → 261	617	21
9-HODE	295 → 171	15	0.50
13-HODE	295 → 195	30	1.0
9-oxoODE	293 → 185	47	1.6
13-oxoODE	293 → 113	29	1.0

^aLOD was defined as a peak whose signal-to-noise ratio is at 3:1.

^bLOQ was defined as a peak whose signal-to-noise ratio is at 10:1