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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Nature. 2009 Sep 20;461(7265):762–767. doi: 10.1038/nature08398

Figure 4. Peptide mimicking repressive marks stimulates PRC2 activity.

Figure 4

(A) Left, coomassie blue staining of reconstituted PRC2-Ezh2 complex. Right, HMT assay with PRC2-Ezh2 alone or in the presence of 10 and 40 μM H3K27, unmodified, mono, di or tri methylated peptides. (B) Titration of the methyl donor (S-adenosyl-Methionine) in the presence of H3K27me0/1/2/3 peptides (C) Nucleosome titration in the presence of H3K27me0/3 peptides. (D) Left, Coomassie blue staining of reconstituted PRC2-Ezh2 Tyr365Ala complex and right, HMT assay with the corresponding complex in the same condition as (A) (E) Relative PRC2 histone methyltransferase activity in the presence of various peptides as indicated in the legend. (F) Table indicating the peptides used for the stimulation study as well as their Kd values (μM) for ΔEed binding (G) Relative PRC2 histone methyltransferase activity in the presence of various peptides as indicated in the legend.