Table 1.
Binding specificities of MBT proteins
| Protein | Peptides | Method | Reference |
|---|---|---|---|
|
dSCM-2MBT (aa 174–435, R277C) |
H3K4me1 (aa 2–15) | ITCa | Grimm et al., 2007 |
| H3K9me1 (aa 2–15) | |||
| H3K27me1 (aa 18–35) | |||
| H3K36me1 (aa 28–44) | |||
| H4K20me1 (aa 12–27) | |||
| hSCML2-2MBTb | H1bmeb | NMRc | Santiveri et al., 2008 |
| H3K4meb | |||
| H3K9meb | |||
| H3K27meb | |||
| H3K36meb | |||
| H4K20meb | |||
| hL3MBTL1-3MBT | H1bK26me1/2 (aa 20–37) | In vitro pull down | Trojer et al., 2007 |
| H4K20me1/2 (aa 16–25) | |||
|
hL3MBTL1-3MBT (aa 200–522) |
H4K20me1/2 (aa 17–25) | ITC | Min et al., 2007 |
| H1bK26me1/2 (aa 21–32) | |||
| H3K4me1/2 | |||
| H3K9me1/2 | |||
| H3K27me1/2 | |||
|
hL3MBTL1-3MBT (aa 206–519) |
H1.4K26me1/2 (aa 18–32) | Fluorescence polarization |
Li et al., 2007 |
| H3K4me1/2 (aa 1–15) | |||
| H3K9me1/2 (aa 1–15) | |||
| H3Kme1 scrambled | |||
| H3K27me1/2 (aa 19–35) | |||
| H3K36me1/2 (aa 28–43) | |||
| H4K20me1/2 (aa 12–27) | |||
| H4Kme1 scrambled | |||
|
hL3MBTL2-4MBT (aa 170–625) |
H3K4me1 | ITC | Guo et al., 2009 |
| H3K9me1/2 | |||
| H3K27me1/2 | |||
| H4K20me1/2 (aa 15–25) | |||
|
dSFMBT-4MBT (aa 531–980) |
H3K9me1/2 (aa 1–15) | Fluorescence polarization |
Klymenko et al., 2006 |
| 3K9me3 (aa 1–15) | |||
| H4K20me1/2 (aa 12–27) | |||
|
dSFMBT-4MBT (aa 535–977) |
H3K4me1/2 (aa 1–15) | ITC Fluorescence polarization |
Grimm et al., 2009 |
| H3K9me1/2 (aa 1–15) | |||
| H3K27me1/2 (aa 19–35) | |||
| H3K36me1/2 (aa 28–43) | |||
| H4K20me1/2 (aa 12–27, 17- 23) | |||
| H4Kme1 scrambled | |||
a
ITC, Isothermal Titration Calorimetry
b
The authors do not indicate the degree of methylation of the peptides or the fragment of hSCML2 used
c
NMR, Nuclear Magnetic Resonance