Figure 3.

Amino acid content of immunoisolated GVs and SVs. A, Astroglial LSS, Sb2-containing vesicles immunoisolated at 21°C and 0°C with or without RgDAAO treatment were analyzed by CE. Electropherograms are adjusted to the same scale to facilitate the comparison between traces. Arrows point to the position of l-serine (lS), d-serine (dS), GABA + unknown substance (γ*), glycine (G), and l-glutamate (E). B, Quantification of amino acid content of GVs and SVs isolated at 0°C. All values were corrected for nonspecific adsorption as determined by isolating vesicles at 21°C. The figure shows mean ± SEM values of four independent experiments, normalized to Sb2 as a vesicle marker.