Figure 5.

Preembedding immunogold staining for d-serine at glia–neuron interfaces in rat cerebral cortex. A, Low-magnification shows that immunogold-positive particles (arrows) are detected in astrocytic processes surrounding asymmetric synapses. High magnifications reveal that d-serine is associated with vesicle-like structures located in astrocytic processes facing the asymmetric synapses. Please note that the insets are rotated compared with the low-magnification image. B, Representative low-magnification (top) and high-magnification (bottom) electron micrographs showing that no immunogold-positive particle is detected with a preabsorbed anti-d-serine antibody. Ast, Astrocyte; D, dendrite; S, dendritic spine; B, axonal bouton. Scale bars, 500 nm.