Figure 6.

Characterization of immunoisolated SVs. A, Cortex homogenate (Input) and bead-bound material (IP) were immunoblotted for Sb2, V-ATPase (subunit a1), synaptophysin, synaptotagmin 1, and for the endosomal marker EEA1. Equal proportion of samples was loaded to allow direct comparison. Light and heavy chains correspond to light and heavy chains of antibodies. Ctl, Control. B, Cortex homogenate, Sb2-containing SVs immunoisolated at 21°C and 0°C were analyzed by CE–LIF. Electropherograms are adjusted to the same scale to facilitate the comparison between traces. Arrowheads point to the position of l-serine (lS), d-serine (dS), GABA (γ), glycine (G), and l-glutamate (E). Quantification of amino acid content in SVs is presented in Figure 3B for comparison with GVs.