Figure 8.

SVs transport l-glutamate but not d-serine. A, Photometric trace showing the absence of d-serine-induced acidification of SVs, whereas l-glutamate can acidify these organelles (10 mm each, n = 3). Scale bar, 2 arbitrary units per 1 min. B, ATP-dependent uptake of d-[³H]serine and l-[³H]glutamate by isolated SVs. The values represent FCCP-sensitive uptake. N.D., Not detectable. Values represent mean ± SEM of two independent experiments performed in triplicate. C, Western blot analysis of cortex homogenate (Input) and control (Ctl) or Sb2 immunoisolates (IP) for vGlut1 and vGlut2 and for SR.