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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Nature. 2012 Jul 26;487(7408):496–499. doi: 10.1038/nature11218

Figure 1. Cell divisions are spatially regulated in the hair follicle at the beginning of a new growth.

Figure 1

a, A hair follicle in quiescence. Distinct populations of cells that participate in hair regeneration, including stem cells, progeny and mesenchyme, reside in defined anatomical compartments of the hair follicle. b, A three-dimensional reconstruction of quiescent live hair follicles from serial optical sections acquired by two-photon laser scanning microscopy. Epithelial nuclei (green) are made visible using the H2B–GFP fusion protein driven by the keratin 14 promoter (K14H2BGFP). c, An example of progeny cell division. A single optical section of a live hair follicle (left panel) and magnified views (right, insets) of three nuclei in the progeny compartment that undergo mitosis in real time (right). d, The locations and axes of cell divisions were quantified from several hair follicles (n = 17) at early growth phase (anagen II). e, Two examples of vertical (left panel) and horizontal (right panel) stem cell divisions. A single optical slice of live hair follicles (left, insets) and magnified views of nuclei in the stem cell compartment (right, insets) undergoing mitosis. Scale bars, 20 µm.