Table 2.

Parameters for PCR screening of floxed GR and FBGRKO mice.

	Cre Recombinase	Germline deletion of floxed GR allele	Homozygosity of floxed GR allele
5' primer	AAGTGCCTTCTCTACA CCTG*	CTCCTCCATTTTGCGA GC	AATCAGAATTGCTCAC TCACAA*
3' Primer	TGCTTATAACACCCTG TTACG*	AAGGAGCAGTCACCA ACTGTC	CAGTGTTACTACTTCC AGTTC*
Mouse CRH Internal Control Primer, 5'	GCTCAGCAAGCTCACA GCAA*	AAACGGAGTAAGGGC AGGAA
Mouse CRH Internal Control Primer, 3'	ACTGGATGACTCCCAT CTGCT	CTCCGGCTGCAAGAA ATTCA
Cycles	1 min, 95° 1 min, 52° 1 min, 72°*	1 min, 95° 1 min, 55° 1 min, 72°	1 min, 95° 1 min, 55° 1 min, 72°*
Cycle number	33*	30	30*

PCR products, along with appropriate positive and negative controls, were separated on 1% (Cre recombinase) or 1.2% agarose gels (other PCR reactions).

^*communicated by Louis Muglia (University of Cincinnati, Cincinnati, OH).