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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Brain Res. 2013 May 28;1525:1–15. doi: 10.1016/j.brainres.2013.05.031

Table 4.

Plasma corticosterone (μg/dl) and ACTH (pg/ml) in 6-month-old C57BL/6 floxed GR and FBGRKO-T29-1 mice.

Floxed GR FBGRKO-T29-1
AM plasma corticosterone (μg/dl) 0.59 ± 0.17 N = 6 0.84 ± 0.28 N = 6
PM plasma corticosterone (μg/dl) 12.4 ± 2.2 N = 6 15.3 ± 1.9 N = 6
PM plasma ACTH (pg/ml) 134.4 ± 31.1 N = 6 161.5 ±19.6 N = 6
Plasma ACTH post-TST (pg/ml) 448.2 ± 47.2 N = 7 463.4 ± 81.1 N = 7
Plasma corticosterone 30 min after restraint (μg/dl) 63.4 ± 13.0 N = 9–10 40.5 ± 6.2 N = 9–10
Plasma corticosterone 120 min after restraint (μg/dl) 49.0 ± 3.8 N = 9–10 46.3 ± 5.6 N = 9–10

Circadian nadir (AM) and peak (PM) plasma corticosterone was measured in samples collected within 2h of lights-on and 1h of lights-off, respectively. Plasma ACTH was measured in the same samples as PM corticosterone and also after an 8 min tail suspension test (TST). Plasma corticosterone after 30 min restraint was measured in samples collected by tail nick immediately (30 min) and 90 min after release (120 min). All other blood samples were collected via submandibular venipuncture.