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Evolutionary conserved positions of amino acids are found at the protein-protein interface of OmpF. a) Conservation entropy (in bits) of transmembrane residues. For illustration, we show Rk = log2 20 − Hk, where well conserved columns show longest bars. The red box highlights a well-conserved structurally contiguous surface patch at the oligomerization interface (strands S2 and S3). b) and c) Structural locations of strands S2 and S3 in the trimer and monomer, respectively.
