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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Breast Cancer Res Treat. 2009 Mar 24;120(1):35–45. doi: 10.1007/s10549-009-0363-8

Fig. 2.

Fig. 2

Expression of SULT1E1 mRNA and protein in the MCF10A-derived lineage cell culture model for breast cancer progression and in MCF7 cells. a A TaqMan Gene Expression assay was used to determine the relative levels of SULT1E1 mRNA in subconfluent (70%, white bars) and confluent (100%, black bars) MCF10A series cells (from left to right, MCF10A, MCF10AT1, MCF10AT1K.cl2, MCF10CA1a, MCF10CA1d and MCF10DCIS.com) and in MCF7 cells. SULT1E1 mRNA content is expressed relative to the level measured in subconfluent MCF10A cells, and all values represent the mean ± SEM of three independent cell culture experiments. Groups labeled with different letters are significantly different from each other (P < 0.05). b Representative Western blots showing the relative amounts of SULT1E1 immunoreactive protein in subconfluent MCF10A-derived cell lines