Fig. 5.

Effects of TSA treatment on ERα and ERβ mRNA expression in MCF10A series and MCF7 cell lines and on E2-mediated activation of an estrogen-responsive reporter gene. a Cell lines were treated for 24 h with 0.1% DMSO (white bars) or 300 ng/ml TSA (black bars), and ERα and ERβ mRNA levels were measured with TaqMan Gene Expression assays. ERα and ERβ mRNA levels are expressed relative to the amounts measured in DMSO-treated MCF10A cells. All values represent the mean ± SEM of three independent cell culture experiments. *, ** Significantly different from the corresponding DMSO-treated group, P < 0.05 and P < 0.01, respectively. b MCF7 cells were transiently transfected with an estrogen-responsive luciferase reporter plasmid. After transfection, cells were treated with 0.1% DMSO, 10 nM E2, 300 ng/ml TSA, or E2 and TSA in combination for 48 h. After treatment, cells were harvested for the measurement of luciferase activities. Each bar represents the mean ± SEM of normalized (firefly/Renilla) luciferase measurements (5 wells per treatment group) relative to the activity measured in DMSO-treated cells. ** Significantly different from the E2-treated group P < 0.01